Publications of 2022

Antibiotics and High-Fat-Diet Induced Gut Inflammation and Impaired Gut Permeability are Improved by Dietary Blueberries Possibly Through NFκB Signaling

Ceres Mattos Della Lucia, Chrissa Petersen, Satheesh Babu Adhini Kuppuswamy, Anandh Babu Pon Velayutham
The FASEB Journal, v. 36.
Impcat factor: 5.191.

abstract: Background: Evidence indicates that a high fat diet can promote gut inflammation and increase gut permeability. The use of antibiotics further exacerbates these complications and can lead to the development of inflammatory bowel disease. Blueberries are rich in bioactive components such as anthocyanins. We recently reported the vascular and prebiotic effects of dietary blueberries. However, the effects of blueberries on gut inflammation and permeability are unknown. In the present study, we tested the hypothesis that blueberries improve antibiotics and high-fat-diet (HFD) induced gut inflammation and impaired gut barrier function in C57BL/6J mice. Methods: 7-week-old male mice (Jackson Lab) consumed standard diet (10% kcal fat) (CON) or HFD (60% kcal fat) with antibiotic cocktail (ampicillin, vancomycin, neomycin sulfate and metronidazole) in drinking water (HFA) or 3.7% freeze-dried blueberry powder supplemented HFD with antibiotic cocktail in drinking water (HFA+BB) for 12 weeks. Body weight and body composition were assessed at the end of the experiment. Inflammation and permeability of the colon were assessed by the mRNA expression of inflammatory markers (IL-1β, IL-6, iNOS, MCP-1); gut barrier markers (MUC-2, cldn-1 and TJP-10); and markers of NFκB signaling (p65, IKK- β and IκBα) by qPCR using specific primers and SYBR green. Results: HFA mice had an increased body weight, reduced lean body mass, and increased body fat compared to CON mice. Blueberry supplementation did not alter these metabolic parameters in HFA+BB vs HFA mice. mRNA expression of inflammatory markers (IL-1β, iNOS, MCP-1); gut barrier marker MUC-2; and markers of NFκB signaling (p65 and IκBα) were increased in HFA vs CON mice. However, blueberry supplementation improved inflammation and permeability of the colon as shown by a reduced colonic expression of IL-1β, iNOS, MCP-1 and MUC-2 in HFA+BB vs HFA mice. Further, a reduced mRNA expression of p65 and IκBα in HFA+BB vs HFA mice indicates that the protective effect of blueberry on the colon could be mediated through NFκB signaling. Conclusion: Blueberry supplementation suppresses colon inflammation and ameliorates colon permeability possibly through NFκB Signaling. Consumption of blueberry is a potential dietary approach to improve gut health.

Taiane Gonçalves Novaes, Larissa Loures Mendes, Luciene Fátima Fernandes Almeida, Andréia Queiroz Ribeiro, Bruna Vieira de Lima Costa, Rafael Moreira Claro, Milene Cristine Pessoa
Ciência e Saúde Coletiva, v. 27, p.2373-2383
Impact factor: 1.336

Abstract: The aim of this study was to analyze the availability of food stores in the territory of schools. Ecological study conducted in Viçosa, Minas Gerais, Brazil, with all schools (N=42) and food stores (N=656). Data were collected through the objective evaluation of the environment, and the stores were categorized into healthy, unhealthy, mixed and supermarkets. Bivariate Ripley´s K function assessed the existence of clustering of categories of stores in the territory of schools. All the schools had at least one food store in their territory. Unhealthy stores were the most common and closest to the schools. There were more stores around private schools, offering high school education, located in the central region and in the highest per capita income tercile. The bivariate Ripley´s K function showed evidence of clustering of stores at all analyzed distances (400 to 1.5 km) with up to 3 times more establishments than would be expected if they were randomly distributed. Therefore, schoolchildren were likely exposed to unhealthy food environments, regardless of neighborhood income and location, which may contribute to inadequate food choices

Black corn (Zea mays L.) whole flour improved the antioxidant capacity and prevented adipogenesis in mice fed a high-fat diet

Thaisa Agrizzi Verediano, Cintia Tomaz Sant’ Ana, Mariana Grancieri, Vinícius Parzanini Brilhante de São José, Renata Celi Lopes Toledo, Maria Cristina Dias Paes, Hércia Stampini Duarte Martino
Food & Function, v. 13, p. 5590-5601
Impact factor: 5.396

Abstract: Black corn (Zea mays L.) is a source of anthocyanins, which have shown the ability to reduce metabolic disorders. This study investigated the anti-inflammatory, antioxidant, and anti-adipogenic preventive effects of black corn. C57BL/6 mice were divided into 3 groups (n = 10): normal control (NC): AIN-93 M; high-fat diet (HF); HF + corn (20%) (HFC). Black corn improved the antioxidant status, through the superoxide dismutase hepatic levels and serum total antioxidant capacity. Animals fed an HFC diet showed decreased gene expression of sterol regulatory element-binding protein-1c (SREBP-1c) and peroxisome proliferator-activated receptor-γ (PPARγ) and increased gene expression of adiponectin and lipoprotein lipase in the adipose tissue, which led to a less inflammatory infiltrate and decreased the adipocyte number and length. In the liver, black corn reduced the gene expression of SREBP-1c and acetyl CoA carboxilase 1. Therefore, black corn whole flour improved the antioxidant capacity, contributed to hepatic β-oxidation, and decreased adipogenesis in animals.

Thaisa Agrizzi Verediano, Hércia Stampini Duarte, Nikolai Kolba, Yimin Fu, Maria Cristina Dias Paes, Elad Tako
Food Research International, v.157, p. 1-12
Impact factor: 6.475

Abstract: Black corn (Zea mays L.) is a pigmented type of this cereal whose color of the kernels is attributed to the presence of the anthocyanins. In this study, we assessed the black corn soluble extract (BCSE) effects on the intestinal functionality, morphology, and microbiota composition using an in vivo model (Gallus gallus) by an intraamniotic
administration. The eggs were divided into four groups (n = 6–10): (1) No Injection; (2) 18 MΩ H2O/cm; (3) 5% (5 mg/mL) BCSE; (4) 15% (15 mg/mL) BCSE. The BCSE showed anti-inflammatory effects bydown regulating the gene expression of tumor necrosis factor-alpha (TNF-α), interleukin 6 (IL6), and the transcriptional nuclear factor kappa beta (NF-κB). Further, the BCSE increased the relative abundance of E. coli and Clostridium. 5% and 15% BCSE increased the hepatic glycogen and upregulated the gene expression of sodiumglucose transport protein (SGLT1). In the morphology, 5% and 15% BCSE increased the goblet cell (GC) number on the crypt, the GC size on the villi, Paneth cell number on the crypt, and the acid GC. Further, the BCSE strengthened the epithelial physical barrier through upregulating the intestinal biomarkers AMP- activated protein kinase (AMPK) and caudal-related homeobox transcriptional factor 2 (CDX2). The overall result suggests that the BCSE promotes intestinal anti-inflammatory effects as well as enhances the intestinal barrier function.

Ana Paula Silva Caldas, Daniela Mayumi Usuda Prado, Ana Paula Dionísio, Helen Hermana Miranda Hermsdorff, Josefina Bressan
British Journal of Nutrition, v. 2022, p.1-11
Impact factor: 3.718

Abstract: Several mechanisms have been proposed for the beneficial effect of nuts on health. However, Brazil and cashew nuts remain the least studied. We aim to evaluate the effect of these nuts within an energy-restricted diet on body weight, body composition, cardiometabolic markers, and endothelial function in cardiometabolic risk women. Brazilian nuts study is a randomized controlled parallel 8-week dietary intervention trial. Forty women were randomly allocated to 1) Control group: Energy-restricted diet without nuts, n= 19 or, 2) Brazil and cashew nuts group (BN-Group): Energy-restricted diet containing daily 45 g of nuts (15 g of Brazil nuts + 30g of cashew nuts), n= 21. At the beginning and final intervention, anthropometry, body composition, and blood pressure were measured. Fasting blood sampling was obtained to evaluate lipid profile, glucose homeostasis, and endothelial function markers. After 8-week, plasma selenium concentration increased in BN-group (∆ = + 31.5 ± 7.8 μg/L; p= 0.001). Brazil and cashew nuts intake reduced total body fat (-1.3 ± 0.4 %) parallel to improvement of lean mass percentage in BN-group compared to the control. Besides, the soluble adhesion molecule VCAM-1 decreased (24.03 ± 15.7 pg/mL vs. -22.2 ± 10.3 pg/mL; p= 0.019) after Brazil and cashew nuts intake compared to the control. However, lipid and glucose profile markers, apolipoproteins, and blood pressure remained unchanged after the intervention. Thus, the addition of Brazil and cashew nuts to an energy-restricted diet can be a healthy strategy to improve body composition, selenium status, and endothelial inflammation in cardiometabolic risk women.

Aline Rosignoli Conceição, Gabriela Macedo Fraiz, Daniela Mayumi Usuda Prado Rocha, Josefina Bressan
Nutrition Research, v.102, p.45-58
Fator de impacto: 3.315

Resumo: The effect of avocado (Persea Americana) on weight loss in people with excess body weight remains unclear. Therefore, we aimed to test our hypothesis that the intake of avocado pulp may be a good strategy for improving anthropometric parameters and, consequently, metabolic health. For this systematic review and meta-analysis, we searched MEDLINE/PubMed, EMBASE, and the Cochrane Central Register of Controlled Trials (CENTRAL) for studies published between database inception and July 2021. Randomized clinical trials (RCTs) assessing avocado intake on anthropometric parameters (primary outcome) and metabolic parameters (secondary outcomes) were included. Evidence from RCTs were synthesized as differences between standardized mean differences (SMDs) for change in body weight and body composition, comparing the experimental avocado group with control via random-effects meta-analyses. The risk of bias followed the Joanna Briggs Institute Reviewer’s Manual. From 781 records identified, 8 studies with 657 individuals were included. No significant changes in body weight, body mass index, percent of body fat, or visceral adipose tissue in response to intervention was seen in the avocado group compared with the control group. Also, the pooled results showed no reduction in body weight and composition (SMD = 0.09; 95% confidence interval, 0.06-0.25; P= .25; I2 = 0%) of avocado in comparison to control. Secondary outcomes showed some potential benefits in metabolic parameters, mainly related to lipid profile. Regardless, consumption of avocado did not promote weight gain, and further studies are needed to elucidate this effect.

Luiza de Paula Dias Moreira, Bárbara Nery Enes, Vinícius Parzanini Brilhante de São José, Renata Celi Lopes Toledo, Luiz Carlos Maia Ladeira, Rodrigo Rezende Cardoso, Vinícius da Silva Duarte, Helen Hermana Miranda Hermsdorff, Frederico Augusto Ribeiro de Barros, Hércia Stampini Duarte Martino
Foods, v.11, p.1-18
Impact factor: 4.350

Abstract: We hypothesized that the consumption of chia (Salvia hispanica L.) flour (CF) and chia oil (CO) improves metabolic disorders in the liver of Wistar rats (Rattus norvegicus domestica) fed a high-fat and high-fructose (HFHF) diet. The animals were fed a HFHF diet (n = 30) or AIN93-M standard diet (n = 10) for eight weeks. After this period, the animals fed HFHF were divided into three groups (n = 10): HFHF diet, HFHF plus 14.7% of CF, and HFHF plus 4% of CO. Histological and biochemical analyses, gene expression, protein levels related to inflammation, and oxidative stress were evaluated in the liver. The HFHF diet caused lipogenesis, liver steatosis, oxidative stress, and inflammation in the animals. The CF and CO intake increased the liver total antioxidant capacity and superoxide dismutase, decreased nitric oxide levels and liver steatosis. Furthermore, the CF and CO led to the upregulation of Cpt1a and Adipor2, respectively, whereas CF downregulated Srebf1. CO intake decreased blood glucose, triglycerides, and the animals’ body weight. Chia did not show effects on mitigating liver pro-inflammatory status, which it may indicate occurs later. The addition of chia into an unbalanced diet is a good and relevant strategy to reduce liver metabolic disorders caused by the high consumption of fructose and saturated fat.

Cooked common bean flour, but not its protein hydrolysate, has the potential to improve gut microbiota composition and function in BALB/c mice fed a high-fat diet added with 6-propyl-2-thiouracil

Mariana Juste Contin Gomes, Juliana Soares da Silva, Natalia Elizabeth Galdino Alves, Andressa de Assis, Elvira Gonzalez de Mejía, Hilário Cuquetto Mantovani, Hércia Stampini Duarte Martino
The Journal of Nutritional Biochemistry, v.106, p.1-13
Impact factor: 6.048

Abstract: Common bean has the potential to improve gut microbiota function due to its chemical composition and content of dietary fiber. This study evaluated the effect of cooked common bean (CCB) flour and its protein hydrolysate as part of a high-fat diet (HFD) added with 6-propyl-2-thiouracil (10 mg/kg/d), an inhibitor of thyroid hormone synthesis, on gut health of BALB/c mice. Forty-eight adult mice were divided into four groups: normal control; HFD; HFD plus CCB flour (346.6 g/kg of diet) (HFBF group) and HFD plus CCB protein hydrolysate (700 mg/Kg/d) (HFPH group). HFBF, but not HFPH, increased cecum weight, and the moisture, and lipids in the excreted feces, compared to control groups. Sequencing of the 16S rRNA gene of the cecal microbiota indicated changes in the beta-diversity between the HFBF and HFPH groups, compared to the normal control. The abundance of Bacteroidetes increased and the Firmicutes/Bacteroidetes ratio decreased in the HFBF compared to control groups. However, HFPH was not able to prevent the damage caused by a HFD to the gut bacterial communities. The OTUs enriched by HFBF were mainly assigned to members of the Muribaculaceae family, which shows potential to improve gut health. The intake of CCB flour improved intestinal health and modulated the composition and function of the cecal microbiota, attenuating the effects of the HFD, added wit 6-propyl-2-thiouracil, when fed to BALB/c mice.

Francilene Maria Azevedo, Almeida Abudo Leite Machamba, Aline Carare Candido, Carina Aparecida Pinto, Sílvia Oliveira Lopes, Mariana de Souza Macedo, Sarah Aparecida Vieira Ribeiro, Silvia Eloiza Priore, Sylvia do Carmo Castro Franceschini
Biological Trace Element Research, v. 2022, p. 1-10
Impact factor: 3.738

Abstract: Iodine is a micronutrient essential for maintaining normal body functioning, and the consumption depends on the distribution in the environment, and insufficient or excessive intake results in thyroid dysfunction. The purpose of this review was to evaluate the correlation between iodine concentration in drinking water and the iodine status of the population. The systematic review was conducted following the PRISMA guidelines and was registered at the International Prospective Register of Ongoing Systematic Reviews (CRD42019128308). A literature search was conducted using MEDLINE/PUBMED (National Library of Medicine), LILACS (Latin-American and Caribbean Literature on Health Sciences), and Cochrane Library, June 2021. The quality of the studies was assessed by a checklist for cross-sectional studies developed by Joanna Briggs Institute. The initial search identified 121 articles, out of which ten were included in this systematic review, and five were included in the meta-analysis. Among the articles listed, six adopted cutoff points to classify the iodine content in the drinking water. The study identified median iodine concentration in drinking water from 2.2 to 617.8 μg/L and the correlation between iodine concentration in drinking water and urinary iodine concentration was 0.92, according to meta-analysis. Furthermore, the iodine status was correlated to the iodine content in water. The determination of a cutoff point can contribute to the implementation of iodine consumption control measures.

Digested protein from chia seed (Salvia hispanica L) prevents obesity and associated inflammation of adipose tissue in mice fed a high-fat diet

Mariana Grancieri, Thaisa Agrizzi Verediano, Cintia Tomaz Sant’Ana, Andressa de Assis, Renata Lopes Toledo, Elvira Gonzalez de Mejia, Hercia Stampini Duarte Martino
PharmaNutrition,v. 21, p.1-11

Abstract: Background and aim: Overweight and obesity are associated with the development of several health complications, such as diabetes, cancer, and cardiovascular disease. Chia seed is a rich source of proteins and peptides with potential anti-inflammatory and antioxidant effects. This study aimed to evaluate the effects of digested proteins (DP) from chia seed to prevent adipogenesis and adipose tissue inflammation in mice fed a high-fat diet. Methods and results: C57Bl/6 black mice were fed a high-fat diet plus DP (400 mg/kg of body/day) for 9 weeks. DP from chia seed reduced levels of plasma total cholesterol (−17.5%), LDL (−42.8%), triacylglycerides (−12.3%), % body fat (−26.98%), and waist circumference (−5.5%) in obese mice (p < 0.05). Furthermore, treatment with DP reduced (p < 0.05) adipocytes area, foci of inflammation, levels of p-NF-κB p65, PPARγ, mRNA SREBP1 (sterol regulatory element-binding transcription), and TNF-α. DP also increased mRNA adiponectin on adipose tissue in animals DP-treated, compared with no-DP-treated animals. However, mRNA LPL and HDL levels were not changed (p > 0.05). The peptides from DP had in silico high interaction with metalloproteinase-2. Conclusions: When using this experimental model, DP from chia seed had an anti-inflammatory and anti-adipogenic effect. These results suggest the effectiveness of digested proteins from chia seed against central obesity and its associated inflammation.

Alessandra da Silva, Brenda Kelly Souza Silveira, Helen Hermana Miranda Hermsdorff, Walmir da Silva, Josefina Bressan
Prostaglandins, Leukotrienes and Essential Fatty Acids, v. 181, p. 1-8
Impact factor: 4.006

Abstract: Evidence suggests antioxidant and anti-inflammatory properties of omega-3 polyunsaturated fatty acids (n-3 PUFA). However, the effect of supplementation of this fatty acid profile on the telomere length and the telomerase enzyme activity was not revised yet. The PubMed and Embase® databases were used to search for clinical trials. A total of six clinical trials were revised. Omega-3 PUFA supplementation did not statistically affect telomere length in three out of three studies but affected telomerase activity in two out of four studies. The supplementation increased telomerase enzyme activity in subjects with first-episode schizophrenia. Besides, it decreased telomerase enzyme activity without modulating the effects of Pro12Ala polymorphism on the PPARγ gene in type 2 diabetes subjects. The methodological differences between the studies and the limited number of studies on the theme suggest that further studies are needed to elucidate the effects of n-3 PUFA supplementation on telomere length and telomerase enzyme activity in humans

Effects of dietary fiber on intestinal iron absorption, and physiological status: a systematic review of and clinical studies

Thaisa Agrizzi Verediano, Nikita Agarwal, Mariana Juste Contin Gomes, Hércia Stampini Duarte Martino, Elad Tako
Critical Reviews in Food Science and Nutrition, v. 2020. P. 1-16
Impact factor: 11.176

Abstract: The benefits of dietary fiber on intestinal health have been well established. However, there is no consensus on the dietary fiber effects on mineral absorption. The objective of this systematic review is to discuss the evidence on the dietary fiber effects on iron absorption and iron status-related biomarkers. A comprehensive search of 3 databases: PubMed, Scopus and Web of Science was carried out. We followed the Preferred Reporting Items for Systematic reviews and Meta-Analyses (PRISMA) guidelines, and a total of 32 studies were included with 9 of them clinical studies and 23 in vivo. The studies included assessment of dietary fiber in the form of fructo-oligosaccharides, galacto-oligosaccharides, inulin, pectin, guar gum, oligofructose, xylo-oligosaccharides, and mannan-oligosaccharide. Hemoglobin (n = 21) and fractional iron absorption (n = 6) were the most frequently reported outcomes. The results showed no significant correlations between consumption of dietary fiber to iron absorption/status-related biomarkers. However, the current evidence may not be substantial to invalidate the recommendation of dietary fiber as an agent to improve dietary iron bioavailability, and absorption. In conclusion, there is a need to conduct further clinical trials with long dietary fiber intervention focusing on population at high risk for iron deficiency.

Emily Ferreira, Kíllya Paiva Santos, Glauce Dias da Costa, Tiago Ricardo Moreira, Luíza Delazari Borges, Heloísa Helena Dias, Daniel Souza Santos, Ítalo Augusto Cunha Rodrigues, Mariângela Orlandi Barbiero, Rosângela Minardi Mitre Cotta
Social innovations Journal, v. 12. P. 1-12

Abstract: Background: This systematic review and meta-analysis aims to estimate the evidence of the effectiveness of the active teaching and learning methods in health majors. Methods: We systematically searched four major databases (i.e., PubMed/MEDLINE, LILACS, Scielo and ERIC). This review was according to the PRISMA method and registration in PROSPERO (CRD42018094054). This review included studies to compare active teaching and learning methods to the traditional methods in the different health majors. We consider all original articles published in the databases until April 29th, 2020. Data were analyzed using R software. The pooled estimates with 95% confidence intervals (CIs) were presented using a forest plot. Higgins and Egger’s tests were used to assess heterogeneity and publication bias, respectively. Primary estimates were pooled using a random-effects meta-analysis model. Results: Of the total of 27 identified articles 16 studies met the inclusion criteria and were included in this meta-analysis. The included studies sample size ranged from 18 to 379. The total sample was 4031 undergraduates from four health courses. The combined meta-analysis was 67% (95% CI: 0.13-0.54). Conclusions: Our finding suggested that evidence exists of the effectiveness of the active teaching and learning methods when compared to the traditional methods in the health courses.

Sílvia Oliveira Lopes, Sarah Aparecida Vieira Ribeiro, Dayane de Castro Morais, Elizangela da Silva Miguel, Laís Silveira Gusmão, Sylvia do Carmo Castro Franceschini, Silvia Eloiza Priore
International Journal of Environmental Research and Public Health, v. 19, p.1-10
Impact factor: 3.390

Abstract: The majority of studies on anemia are focused on children and women of reproductive age. Although the disease is a widespread public health problem, studies that include the rural population are scarce. This study determined the prevalence of anemia and associated factors in adults and the elderly living in the rural area of a municipality in Minas Gerais. Twelve rural communities were included. During home visits, hemoglobin levels were measured using a hemoglobinometer to check for the presence or absence of anemia. Additionally, anthropometric data and food insecurity data based on the Brazilian Food Insecurity Scale (EBIA) were collected. A questionnaire about socioeconomic, demographic, and housing conditions was applied. Analyses were performed using the Stata software version 13.0. Spearman correlation and regression analysis logistics were performed (p < 0.05) on 124 families (n = 297 farmers). The prevalence of anemia was 41.1%, being higher among women (55.7%). Additionally, 40.1% of the farmers were food insecure; 52.7% and 80.5% presented excess weight and cardiovascular risk, respectively. Poverty was a reality for 39.7% of individuals. A positive correlation between hemoglobin levels and per capita income was found as well as a negative correlation with EBIA scores and cardiovascular risk. Multivariate analysis showed that individuals experiencing food insecurity, the elderly, and those who do not own a property, were more likely to be anemic. Farmers with per capita income above 1/2 minimum wage were less likely to have anemia. The prevalence of anemia in the group studied was higher than previous studies. The disease is associated with factors that also predispose to food insecurity. The improvement of the determinants of insecurity can contribute to the fight against anemia

Bruna Clemente Cota, Felício Roberto Costa, Leidjaira Lopes Juvanhol, Sarah Aparecida Vieira Ribeiro, Silvia Eloiza Priore, Eliane Rodrigues de Faria, Franciane Rocha de Faria, Patrícia Feliciano Pereira
British Journal of Nutrition, v. 2022
Impact factor: 3.718

Abstrct: A new phenotype of obesity has been studied: normal-weight obesity (NWO), which describes individuals with normal-weight by body mass index (BMI) and excess body fat. Despite normal-weight, individuals with NWO have a higher cardiometabolic risk. There is still a gap in the literature on the subject, especially in adolescents, as studies with this population are scarce. Therefore, this study aims to investigate the sociodemographic factors, family history of chronic non-communicable diseases, body perception, lifestyle and food consumption associated with NWO in adolescents. This is a cross-sectional study, conducted with 506 normal-weight adolescents aged 10 to 19 years, of both sexes. Weight and height were obtained and BMI/age was calculated. Body fat analysis was performed using dual-energy X-ray absorptiometry. Sociodemographic data, level of physical activity, food consumption, body self-perception and lifestyle habits were also obtained. Logistic regression with hierarchical approach was used to analyze the associations. The odds of NWO are greater with age (OR=1.14; 95% CI=1.04-1.26), lower in male adolescents (OR=0.21; 95% CI=0.11-0.41) and higher in those with a history of familial dyslipidemia (OR=1.81; 95% CI=1.01-3.28). Adolescents satisfied with their body (OR=0.30; 95%CI 0.16-0.56) and physically active (OR=0.44; 95% CI=0.24-0.81) have a lower odds of NWO, compared to the others. In addition, it was observed that the odds of NWO is greater among adolescents who use sweeteners (sugar substitutes) (OR=3.84; 95% CI=1.70-8.65). The factors associated with NWO were female sex, older age, positive family

Jerusa da Mota Santana, Marcos Pereira, Gisele Queiroz Carvalho, Maria do Carmo Gouveia Peluzio, Iúri Drumond Louro, Djanilson Barbosa Dos Santos, Ana Marlucia Oliveira
Nutrients, v.14, p. 1 -13
Impact factor: 5.717

Abstract: The polymorphisms of fatty acid desaturase genes FADS1 and FADS2 have been associated with an increase in weight gain. We investigated FADS1 and FADS2 gene polymorphisms and the relation between ω-3 and ω-6 fatty acid plasma concentrations and gestational weight gain. A prospective cohort study of 199 pregnant women was followed in Santo Antônio de Jesus, Brazil. Plasma levels of polyunsaturated fatty acids (PUFAs) were measured at baseline and gestational weight gain during the first, second, and third trimesters. Fatty acid recognition was carried out with the aid of gas chromatography. Single nucleotide polymorphisms (SNPs) were genotyped using real-time PCR. Statistical analyses included Structural Equation Modelling. A direct effect of FADS1 and FADS2 gene polymorphisms on gestational weight was observed; however, only the SNP rs174575 (FADS2) showed a significant positive direct effect on weight over the course of the pregnancy (0.106; p = 0.016). In terms of the influence of SNPs on plasma levels of PUFAs, it was found that SNP rs174561 (FADS1) and SNP rs174575 (FADS2) showed direct adverse effects on plasma concentrations of ω-3 (eicosapentaenoic acid and alpha-linoleic acid), and only SNP rs174575 had positive direct effects on plasma levels of ARA and the ARA/LA (arachidonic acid/linoleic acid) ratio, ω-6 products, while the SNP rs3834458 (FADS2) had an adverse effect on plasma concentrations of EPA, leading to its increase. Pregnant women who were heterozygous and homozygous for the minor allele of the SNP rs3834458 (FADS2), on the other hand, showed larger concentrations of series ω-3 substrates, which indicates a protective factor for women’s health.

Food Craving and Its Associated Factors during COVID-19 Outbreak in Brazil

Lívya Alves Oliveira, Ceres Mattos Della Lucia, Fabiane Aparecida Canaan Rezende, Lívia Garcia Ferreira, Lucilene Rezende Anastácio, Tamires Cássia de Melo Souza, Marina Martins Daniel, Juliana Costa Liboredo
American Journal of Health Education, v. 53, p. 207-218

Abstract: Background: Social distancing used as a measure to contain the spread of SARS-CoV-2, led to a series of changes in eating behavior, including food craving. Purpose: We aimed to evaluate food craving and its associated factors among women and men during the COVID-19 pandemic. Methods: An online questionnaire was conducted, containing data about food craving and possible associated factors. Results: 1363 individuals were included in the survey. Food craving was shown in 46.0% of the respondents. Among women, increased or reduced weight, working/studying during the pandemic without changes, increased snacking, worsened sleep quality, uncontrolled eating were some factors associated with food craving. For men, some factors associated were having a morning snack, living with parents, reduced sleep hours, and emotional eating. Discussion: Factors associated with food craving varied between men and women, being more present among women. These differences can be related to hormonal differences, way of working, daily tasks, and food preferences. Translation to Health Education Practice: Our findings may be used to develop guidelines for maintaining healthy eating habits in pandemic situations. In addition, the study showcases the need for health educators to consider the qualitative and quantitative aspects of nutrition, which can interfere with increased food cravings.

Andressa Ladeira Bernardes, Luís Fernando de Sousa Moraes, Bruna Cristina dos Santos Cruz, Lisiane Lopes da Conceição, Leandro Licursi de Oliveira, Mariaurea Matias Sarandy, Reggiani Vilela Gonçalves, Maria do Carmo Gouveia Peluzio
British Journal of Nutrition, v. 2022, p.1-12
Impact factor: 3.718

Abstract: The development of colorectal cancer involves some morphological changes, and in the initial stage, pre-neoplastic lesions called aberrant crypt foci (ACF) appear. Thus, an intervention with sources of bioactive compounds such as Hibiscus sabdariffa L., rich in phenolic compounds and anthocyanins, could attenuate the risk of developing these lesions due to its antioxidant, anti-inflammatory and anti-proliferative properties. Therefore, the aim of this study was to evaluate the effects of 5 % and 10 % supplementation of dehydrated H. sabdariffa calyces (DHSC) during the development of 1,2-dimethylhydrazine-induced pre-neoplastic lesions in male BALB/c mice. The characterisation of DHSC was carried out. The in vivo experiment lasted 12 weeks, and the animals were randomly divided into three experimental groups: the control group (CON) and the supplemented groups with 5 % DHSC and 10 % DHSC. The activities of liver enzymes catalase (CAT) and superoxide dismutase were determined. In addition, ACF, SCFA, presence of inflammatory infiltrates, goblet cells and leucocytes in the colonic mucosa were quantified. There was a significant reduction in ACF and the presence of inflammatory infiltrates in the colon of animals in groups 5DHSC and 10DHSC. In addition, the 10DHSC group showed an increase in the activity of the CAT enzyme, in the production of butyrate and in the presence of natural killer cells in the colon, in addition to more hypertrophied goblet cells. Based on these findings, it is suggested that DHSC supplementation may be recommended to attenuate cellular responses in the early stage of pre-neoplastic lesions.

Marina Martins Daniel, Juliana Costa Liboredo, Lucilene Rezende Anastácio, Tamires Cássia de Melo Souza, Lívya Alves Oliveira, Ceres Mattos Della Lucia, Lívia Garcia Ferreira
Frontiers in Nutrition, v.2022, p.1-11
Impact factor: 6.576

Abstract: Introduction and aims: The behavioral changes that arose from quarantine due to the COVID-19 pandemic may have impacted the weight of people. This study aims to investigate the incidence and predictors of weight gain during the quarantine period. Methods: An online survey was performed five months after the social distance measures implementation. Participants recorded their current and usual weight before lockdown. A multivariate logistic regression model was performed. Results: Data on 1334 participants were evaluated (33.6y, 79.8% females), and 58.8% have gained weight (3.0 kg; 0.1 to 30.0 kg). Predictors of weight gain were increased food intake (OR = 5.40); snacking (OR = 2.86); fast food (OR = 1.11); canned products (OR = 1.08); and in physical activity (OR = 0.99) concerning the period before the pandemic; also time spent at work, including household chores (OR = 1.58); evening snack (OR = 1.54); higher frequency of alcoholic beverage intake (OR = 1.59) and dose of alcoholic beverage (OR = 1.11); uncontrolled eating (OR = 1.01), and vegetable intake (OR = 0.92) during the quarantine and physical activity before pandemic period (OR = 0.99). Conclusion: Most participants have gained weight during the pandemic because of working changes, lifestyle, eating habits changes, and uncontrolled eating behavior. These results can be useful to encourage changes during future quarantine periods to prevent weight gain.

Lara Gomes Suhett, Leidjaira Juvanhol Lopes, Mariane Alves Silva, Sarah Aparecida Vieira Ribeiro, Helen Hermana Miranda Hermsdorff, Nitin Shivappa, James R Hébert, Juliana Farias de Novaes
Nutrition, v. 2022, p. 1-20
Impact factor: 4.008

Abstract: Objectives: Missed nutrients from skipped meals affect diet quality. However, the extent to which breakfast skipping affects the inflammatory potential of a diet, as indicated by Children’s Dietary Inflammatory Index (C-DII) score, remains unknown. We aimed to evaluate the association between breakfast skipping and C-DII score, and investigate the presence of interaction with sociodemographic factors and sedentary behavior. Methods: This representative cross-sectional study enrolled 378 children ages 8 and 9 y from Viçosa, Minas Gerais, Brazil in 2015. We collected sociodemographic data (sex, age, race, and household per-capita income) and screen time using a semistructured questionnaire. Dietary intake and breakfast skipping were evaluated by three 24-h dietary recalls from which energy-adjusted C-DII scores were calculated. We performed linear regression models to test the associations and possible interactions. Results: The prevalence of breakfast skipping and sedentary behavior were 20.1% and 47.6%, respectively. The mean ± standard deviation of the C-DII scores was 0.60 ± 0.94, and ranged from -2.16 (most anti-inflammatory diet) to 2.75 (most proinflammatory diet). Breakfast skipping was associated with a higher intake of lipids, monounsaturated fat, and polyunsaturated fat, as well as lower carbohydrate, calcium, and magnesium intake (P < 0.05). After adjustment, breakfast skippers had higher C-DII scores (β = 0.33; 95% confidence interval, 0.12-0.54). This association was more pronounced in children with sedentary behavior (β = 0.53; 95% confidence interval, 0.24-0.82). Conclusions: Breakfast skipping was associated with a more proinflammatory diet in school-age children, and there was significant interaction with sedentary behavior. Early childhood interventions encouraging the habit of eating a breakfast and engaging in physical activity may help reduce the dietary inflammatory potential and prevent related cardiometabolic disorders.

Elizangela da Silva Miguel, Davi Lopes do Carmo, Sílvia Oliveira Lopes, Ricardo Henrique Silva Santos, Sylvia do Carmo Castro Franceschini, Luiza Carla Vidigal Castro, Glauce Dias da Costa, Silvia Eloíza Priore
Research, Society and Development, v. 11, p.1-12

Abstract: This study aimed to evaluate the association between the use of pesticides, symptoms of intoxication and health conditions of family farmers in the Mata Mineira area, Brazil. This is a cross-sectional study with 48 family farmers from a municipality in the Zona da Mata, Minas Gerais. A semi-structured questionnaire was applied to obtain demographic, socioeconomic information and characterization of the use of pesticides. Blood samples were collected from farmers for biochemical tests and evaluation of markers of exposure to pesticides. It was found that 75% of farmers used pesticides in food production, most of these products being extremely toxic. These farmers showed changes in plasma cholinesterase, erythrocyte cholinesterase and butyrylcholinesterase, markers of exposure to pesticides. There was an association between spraying for more than 4 hours/day and reporting of acute symptoms of intoxication, as well as between the use of pesticides and the presence of chronic non-communicable diseases. Multivariate analysis indicated that non-compliance with the period of re-entry into crops after the use of pesticides remained as an explanatory variable for lung disease. We infer that acute symptoms, as well as chronic non-communicable diseases reported by family farmers, may be associated with the use of pesticides.

Leidjaira Lopes Juvanhol, Aline Silva‑Costa, Lucia Rotenberg, Arlinda B. Moreno, Enirtes Caetano Prates Melo, Itamar S. Santos, Maria Angélica Antunes Nunes, Susanna Toivanen, Dóra Chor, Rosane Härter Griep
Journal of Eating Disordes, v. 2022, p. 1.9
Impact factor: 4.049

Abstract: Background: Job strain has been reported as a trigger for binge eating, yet the underlying mechanisms have been unclear. The aim of this study was to evaluate whether work-family conflict is a pathway in the association between job strain and binge eating, considering the possible effect-modifying influence of body mass index (BMI). Methods: This cross-sectional analysis included 12,084 active civil servants from the multicenter Brazilian Longitudinal Study of Adult Health (ELSA-Brasil). Job strain was assessed using the Demand-Control-Support Questionnaire. Work-family conflict was considered as a latent variable comprising three items. Binge eating was defined as eating a large amount of food in less than 2 h at least twice a week in the last six months with a sense of lack of control over what and how much was eaten. Structural equation modelling was used to test the role of work-family conflict in the association between job strain and binge eating, stratifying for BMI. Results: For individuals of normal weight, positive associations were found between skill discretion and binge eating (standardized coefficient [SC] = 0.209, 95%CI = 0.022-0.396), and between psychological job demands and work-family conflict (SC = 0.571, 95%CI = 0.520-0.622), but no statistically significant indirect effect was found. In overweight individuals, psychological job demands, skill discretion, and work-family conflict were positively associated with binge eating (SC = 0.099, 95%CI = 0.005-0.193; SC = 0.175, 95%CI = 0.062-0.288; and SC = 0.141, 95%CI = 0.077-0.206, respectively). Also, work-family conflict was observed to be a pathway on the associations of psychological job demands and decision authority with binge eating (SC = 0.084, 95%CI = 0.045-0.122; and SC = – 0.008, 95%CI = – 0.015- – 0.001, respectively). Conclusions: Work-family conflict partly explains effects of high levels of psychological job demands and low levels of decision authority on binge eating among overweight individuals. Moreover, skill discretion is positively associated with binge eating, regardless of BMI category.

Low polyphenols intake among highly scholarity population: CUME cohort

Amanda Popolino Diniz, Josefina Bressan, Raquel de Deus Mendonça, Hillary Nascimento Coletro, Júlia Cristina Cardoso Carraro, Adriano Marçal Pimenta, Helen Hermana Miranda Hermsdorff, Adriana Lúcia Meireles
International Journal for Vitamin and Nutrition Research, v. 2022
Impact factor: 1.784

Abstract: In the absence of a recommendation for daily intake of phenolic compounds, to compare the intake of the main dietary polyphenols between populations is a really challenge. This study aimed to estimate the total dietary intake of polyphenols, classes and their food sources among Brazilian graduates and postgraduates. This was a cross-sectional study using baseline data from the Cohort of Universities of Minas Gerais (CUME project). Food consumption was assessed using a quantitative food frequency questionnaire with 144 food items. Polyphenol intake was estimated from the Phenol-Explorer and US Department of Agriculture database, and previous studies that evaluated the phenolic content of specific foods. A total of 4130 individuals of both sexes with a median age of 34 (18–86) years old were finally included. The median intake of total polyphenols adjusted for energy was 753.41 mg/day (interquartile range – IQR=461.80; p<0.001), and the most consumed classes were phenolic acids and flavonoids, with median intakes of 552.30 mg/d (IQR=429.78; p<0.001) and 154.70 mg/day (IQR=108.70; p<0.001), respectively. The main food sources of polyphenols were coffee, peanuts, beans, and fruits. A lower intake of total polyphenols and their classes was observed in a population with similar characteristics to those from developed countries. The results demonstrate the importance of disseminating nutritional information about foods, so that the consumption of natural foods is prioritized. New studies that evaluate the consumption of polyphenols and their impact on human health are recommended to establish a daily recommendation for the consumption of such compounds.

Reggiani Vilela Gonçalves, Mariáurea Matias Sarandy, Débora Esposito, Maria do Carmo Gouveia Peluzio
Oxidative Medicine and Cellular Longevity, v. 2022, p.1-2
Impact factor: 6.543

Retail food outlets and metabolic syndrome: a systematic review of longitudinal studies.

Fernanda Martins de Albuquerque, Milene Cristine Pessoa, Mariana De Santis Filgueiras, Danielle Soares Gardone, Juliana Farias de Novaes
Nutrition Reviews, v.2022
Impact factor: 7.11

Abstract: Context: The community food environment covers the type, quantity, density, location, and access to retail food outlets, and its influence on eating behavior, obesity, and metabolic syndrome has been investigated. Objective: To evaluate the evidence on longitudinal associations between objectively measured retail food outlets and metabolic syndrome components in children, adolescents, and adults. Data extraction: This systematic review followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. Study quality and risk of bias were assessed with the Newcastle-Ottawa Scale. Data sources: The Scopus, Embase, Web of Science, Scielo, PubMed, MEDLINE, and Lilacs databases were searched without any restriction on publication dates. Data analysis: Of the 18 longitudinal studies included, significant associations were reported in 9 between retail food outlets and metabolic syndrome components in adults (6 positive associations, 2 negative, and 1 both positive and negative), and in 3 studies of children and adolescents (2 negative associations and 1 both positive and negative). Six studies with adults found no association. Conclusion: Limited evidence was found for longitudinal associations between retail food outlets and metabolic syndrome components. In future studies, researchers should consider the use of standardized retail food outlet measurements and accurate analysis to better understand the influence of the community food environment on metabolic syndrome.

Daniel Barbosa Alcântara, Ana Paula Dionísio, Adriana G. Artur, Brenda Kelly Souza Silveira, Amanda F. Lopes, Jhonyson A. C. Guedes, Lícia R. Luz, Ronaldo F. Nascimento, Gisele S. Lopes, Helen Hermana Miranda Hermsdorff, Guilherme J. Zocolo
Food Chemistry, v. 137, p.1-17
Impact factor: 7.514

Abstract: Se is an essential element in mammals. We review how its bioavailability in soil and the ability of plants to accumulate Se in foods depends on the soil Se profile (including levels and formats), besides to describe how the various selenoproteins have important biochemical functions in the body and directly impact human health. Owing to its favorable characteristics, the scientific community has investigated selenomethionine in most nut matrices. Among nuts, Brazil nuts have been highlighted as one of the richest sources of bioavailable Se. We summarize the most commonly used analytical methods for Se species and total Se determination in nuts. We also discuss the chemical forms of Se metabolized by mammals, human biochemistry and health outcomes from daily dietary intake of Se from Brazil nuts. These findings may facilitate the understanding of the importance of adequate dietary Se intake and enable researchers to define methods to determine Se species.

Synbiotic modulates intestinal microbiota metabolic pathways and inhibits DMH-induced colon tumorigenesis through c-myc and PCNA suppression

Bruna Cristina dos Santos Cruz, Vinícius da Silva Duarte, Roberto Sousa Dias, Andressa Ladeira Bernardes, Sérgio Oliveira de Paula, Célia Lúcia de Luces Fortes Ferreira, Maria do Carmo Gouveia Peluzio
Food Research International, v. 158, p.1-12
Impact factor: 6.475

Abstract: The use of probiotic and synbiotic is a promising strategy to modulate the intestinal microbiota, and thereby modify the risk of diseases. In this study, the effect of probiotic VSL#3, isolated or associated with a yacon-based product (PBY), on the functional metabolic pathways of the microbiota, in a colorectal carcinogenesis model, was evaluated. For this, mice induced to carcinogenesis were fed with standard diet AIN-93 M (CON), diet AIN-93 M and VSL#3 (PRO) or diet AIN-93 M with yacon and VSL#3 (SYN). The SYN group showed a highly differentiated intestinal community based on the MetaCyc pathways. Of the 351 predicted functional pathways, 222 differed between groups. Most of them were enriched in the SYN group, namely: amino acid biosynthesis pathways, small molecule biosynthesis pathways (cofactors, prosthetic groups, electron carriers and vitamins) carbohydrate degradation pathways and fermentation pathways. In addition, the synbiotic was able to stimulate the anti-inflammatory immune response and reduce the gene expression of PCNA and c-myc. Thus, we conclude that the synbiotic impacted more significantly the metabolic functions of the microbiota compared to the isolated use of probiotic. We believe that the enrichment of these pathways can exert antiproliferative action, reducing colorectal carcinogenesis. The prediction of the functional activity of the microbiota is a promising tool for understanding the influence of the microbiome on tumor development.

The efectiveness of the use of telehealth programs in the care of individuals with hypertension and, or diabetes mellitus: systematic review and meta-analysis

Daniel Souza Santos, Clara Regina Santos Batistelli, Marina Marilac dos Santos Lara, Emily de Souza Ferreira, Tiago Ricardo Moreira, Rosângela Minardi Mitre Cotta
Diabetology & Metabolic Syndrome, v. 2022, p. 1-9
Impact factor: 3.320

Abstract: Introduction: Diabetes Mellitus and Hypertension are some of the main Chronic Noncommunicable Diseases, representing a big challenge for global health. In this context, Telehealth programs are presented as a tool with exciting potential to complement and support health care. This paper aimed to analyze the effectiveness of the use of Telehealth programs in the care of individuals with Hypertension and/or Diabetes Mellitus. Methods: A systematic review with meta-analysis was carried out according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) protocol methodology. The following databases were used: PubMed, EMBASE, SciELO, ScienceDirect and Cochrane Library. Papers were included if they addressed the use of technologies that allow two-way communication at a distance between health professionals and patients affected by Hypertension and/or Diabetes Mellitus, type 1 or type 2. Experimental, cross-sectional, case–control, cohort, and clinical trials were included in the review. Results: We included 164 papers in the review and 45 in the meta-analysis final synthesis. The systematic review results showed a prevalence of telemonitoring as the main form of Telehealth. The study showed a reduction in expenses with the use of Telehealth, both for the users and for the health systems providers, followed by greater satisfaction. Our meta-analysis showed that Telehealth is an effective tool in the care of diabetic patients, providing a 0.353% reduction in HbA1c compared to traditional care. No studies on Hypertension that met our eligibility criteria for inclusion in the meta-analysis were found. Conclusions: Telehealth is an effective tool for the care of people with Diabetes Mellitus and/or Hypertension

Flavia Glavão Cândido, Raquel Duarte Moreira Alves, Dayse Mara de Oliveira Freitas, Jersica Martins Bittencourt, Daniela Mayumi Usuda Prado Rocha, Rita de Cassia Gonçalves Alfenas
Clinical Nutrition ESPEN, v. 2022, p.1-6
Impact factor: 7.324

Abstract: Objective. To investigate if the gout-protective effect of low-fat dairy products could be attributed to the urate-lowering effect of calcium. Methods. This is a placebo-controlled trial in which thirty-five adult (aged 18–42 years) female low-calcium consumers (<800 mg/d) were randomized to one of three treatment groups: low calcium breakfast (control, ∼70 mg of calcium/d) –C or high-calcium breakfast (∼770 mg/d) from calcium citrate – CIT or from skim milk – SM, during 45 consecutive days. Breakfasts were matched for potential confounders and were provided as part of an energy-restricted normoprotein diet containing an additional 800 mg of calcium/d. Dual-energy X-ray absorptiometry measurements (body fat assessment) and fasting blood samples (urate, ionic calcium, PTH, and 1,25-(OH)2-D3) were taken at baseline and the end of the experiment. Clinical trial registration: (RBR-7Q2N33). Results. Despite no significant changes in total body weight/fat, CIT and SM led to a significant reduction in serum urate and ionic calcium, but did not affect PTH and vitamin D concentrations compared to C. CIT and SM reduced baseline serum urate by ∼14% and ∼17%, respectively. There was a trend to a positive correlation between changes in serum urate and changes in ionic calcium on day 45 (r = 0.327, P = 0.055). Conclusions. Calcium supplementation (770 mg/d from dairy or calcium citrate) reduced serum urate concentrations, suggesting that the gout-protective effect of low-fat dairy consumption is at least partly due to a urate-lowering effect of calcium.

Mariana Juste Contin Gomes, Hércia Stampini Duarte Martino, Nikolai Kolba, Jacquelyn Cheng, Nikita Agarwal, Maurisrael de Moura Rocha, Elad Tako
Frontiers in Bioscience Landmark, v.27, p.1-13
Impact factor: 4.009

Abstract: Background: Biofortification is a method that improves the nutritional value of food crops through conventional plant breeding. The aim of this study was to evaluate the effects of intra-amniotic administration of soluble extracts from zinc (Zn) biofortified and Zn standard cowpea (Vigna unguiculata L. Walp.) flour on intestinal functionality and morphology, inflammation, and gut microbiota, in vivo. Methods: Seven treatment groups were utilized: (1) No Injection; (2) 18 MΩ H2O; (3) 50 mg/mL Inulin; (4) 50 mg/mL BRS Pajeú soluble extract (Zn standard); (5) 50 mg/mL BRS Aracê soluble extract (Zn biofortified); (6) 50 mg/mL BRS Imponente soluble extract (Zn biofortified); (7) 50 mg/mL BRS Xiquexique soluble extract (Zn biofortified). Results: Treatment groups with BRS Imponente and BRS Xiquexique reduced the abundance of Clostridium and E. coli when compared with all other experimental groups. All cowpea soluble extracts increased villi goblet cell number (total), specifically acidic goblet cell type number per villi relative to inulin and 18MΩ H2O groups. Moreover, BRS Xiquexique increased the crypt goblet diameter and the crypt depth compared to all treatments and controls. The Zn content in the Zn biofortified cowpea flours was higher when compared to the Zn standard flour (BRS Pajeú), and the phytate: Zn molar ratio was lower in the Zn biofortified flours compared to the Zn standard flour. In general, all cowpea soluble extracts maintained the gene expression of proteins involved with Zn and iron absorption, brush border membrane (BBM) functionality and inflammation compared to inulin and 18MΩ H2O. Conclusions: This study demonstrates the potential nutritional benefit of standard and biofortified
cowpea treatment groups to improve intestinal morphology, BBM functionality, inflammation, and gut microbiota, with the highest effect of BRS Xiquexique soluble extracts to improve assessed cecal microflora populations and intestinal morphology.


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